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Int J Clin Exp Pathol 2013;6(6):1132-1137
Original Article
Correlation of ECM1 expression level with the pathogenesis and metastasis of laryngeal
carcinoma
Meizhen Gu, Jian Guan, Limin Zhao, Kun Ni, Xiaoyan Li, Zhao Han
Department of Otolaryngology, Shanghai Children’s Hospital, Shanghai Jiao Tong University, Shanghai 200040, China; Department of
Otolaryngology, Affiliated Sixth People’s Hospital, Shanghai Jiao Tong University, Shanghai 200233, China; Department of Otolaryngology, Eye
Ear Nose and Throat Hospital, Fudan University, Shanghai 200031, China
Received March 17, 2013; Accepted April 7, 2013; Epub May 15, 2013; Published June 1, 2013
Abstract: Objective: To investigate the expression of extracellular matrix protein 1 (ECM1) in benign laryngeal lesions, precancerous lesions
and malignant laryngeal lesions and analyze the clinical significance of ECM1 changes in the pathogenesis and metastasis of laryngeal
carcinoma. Methods: A total of 46 patients with laryngeal lesions were recruited with a median age of 48.2 years (range: 33-67 years). Among
these patients, 29 had laryngeal carcinoma (12 with metastasis and 17 without metastasis), 8 had benign laryngeal lesions and 9 had
precancerous laryngeal lesions (laryngeal leukoplakia). Immunofluorescence staining was employed to detect the protein expression of ECM1
in benign laryngeal lesions, laryngeal leukoplakia and malignant laryngeal lesions; RT-PCR was used to measure the mRNA expression of
ECM1 in laryngeal carcinoma and benign laryngeal lesions. Results: ECM1 expression was detected in 25% (2/8) of patients with benign
laryngeal lesions, 78% (7/9) of patients with precancerous laryngeal lesions, and 100% (29/29) of patients with laryngeal carcinoma. Among
the laryngeal carcinoma patients, high ECM1 expression (+++) was found in 64.7% (11/17) of patients without lymph node metastasis and
91.7% (11/12) of patients with lymph node metastasis. Increased ECM1 expression was found in laryngeal carcinoma when compared with
other laryngeal lesions and the ECM1expression in patients with metastasis was significantly higher than that patients without metastasis
(P<0.01). RT-PCR showed that the mRNA expression of ECM-1 in laryngeal carcinoma was markedly higher than that in benign laryngeal
lesions. Conclusion: ECM1 expression is in an increasing order in benign laryngeal lesions, precancerous laryngeal lesions and malignant
laryngeal lesions. Meanwhile, the metastatic laryngeal carcinoma has higher ECM1 expression than laryngeal carcinoma without metastasis.
Our findings suggest that ECM1 plays promotive roles in the occurrence, development and metastasis of laryngeal carcinoma.
(IJCEP1303039).
Keywords: Extracellular matrix protein 1, laryngeal carcinoma, lymph node metastasis
Address correspondence to: Dr. Zhao Han, Department of Otolaryngology, Eye Ear Nose and Throat Hospital, Fudan University, Shanghai
200031, China. E-mail: hanye7899@sohu.com; Dr. Xiaoyan Li, Department of Otolaryngology, Shanghai Children’s Hospital, Shanghai Jiao
Tong University, Shanghai 200040, China. E-mail: li6789789@sina.com
Original Article
Correlation of ECM1 expression level with the pathogenesis and metastasis of laryngeal
carcinoma
Meizhen Gu, Jian Guan, Limin Zhao, Kun Ni, Xiaoyan Li, Zhao Han
Department of Otolaryngology, Shanghai Children’s Hospital, Shanghai Jiao Tong University, Shanghai 200040, China; Department of
Otolaryngology, Affiliated Sixth People’s Hospital, Shanghai Jiao Tong University, Shanghai 200233, China; Department of Otolaryngology, Eye
Ear Nose and Throat Hospital, Fudan University, Shanghai 200031, China
Received March 17, 2013; Accepted April 7, 2013; Epub May 15, 2013; Published June 1, 2013
Abstract: Objective: To investigate the expression of extracellular matrix protein 1 (ECM1) in benign laryngeal lesions, precancerous lesions
and malignant laryngeal lesions and analyze the clinical significance of ECM1 changes in the pathogenesis and metastasis of laryngeal
carcinoma. Methods: A total of 46 patients with laryngeal lesions were recruited with a median age of 48.2 years (range: 33-67 years). Among
these patients, 29 had laryngeal carcinoma (12 with metastasis and 17 without metastasis), 8 had benign laryngeal lesions and 9 had
precancerous laryngeal lesions (laryngeal leukoplakia). Immunofluorescence staining was employed to detect the protein expression of ECM1
in benign laryngeal lesions, laryngeal leukoplakia and malignant laryngeal lesions; RT-PCR was used to measure the mRNA expression of
ECM1 in laryngeal carcinoma and benign laryngeal lesions. Results: ECM1 expression was detected in 25% (2/8) of patients with benign
laryngeal lesions, 78% (7/9) of patients with precancerous laryngeal lesions, and 100% (29/29) of patients with laryngeal carcinoma. Among
the laryngeal carcinoma patients, high ECM1 expression (+++) was found in 64.7% (11/17) of patients without lymph node metastasis and
91.7% (11/12) of patients with lymph node metastasis. Increased ECM1 expression was found in laryngeal carcinoma when compared with
other laryngeal lesions and the ECM1expression in patients with metastasis was significantly higher than that patients without metastasis
(P<0.01). RT-PCR showed that the mRNA expression of ECM-1 in laryngeal carcinoma was markedly higher than that in benign laryngeal
lesions. Conclusion: ECM1 expression is in an increasing order in benign laryngeal lesions, precancerous laryngeal lesions and malignant
laryngeal lesions. Meanwhile, the metastatic laryngeal carcinoma has higher ECM1 expression than laryngeal carcinoma without metastasis.
Our findings suggest that ECM1 plays promotive roles in the occurrence, development and metastasis of laryngeal carcinoma.
(IJCEP1303039).
Keywords: Extracellular matrix protein 1, laryngeal carcinoma, lymph node metastasis
Address correspondence to: Dr. Zhao Han, Department of Otolaryngology, Eye Ear Nose and Throat Hospital, Fudan University, Shanghai
200031, China. E-mail: hanye7899@sohu.com; Dr. Xiaoyan Li, Department of Otolaryngology, Shanghai Children’s Hospital, Shanghai Jiao
Tong University, Shanghai 200040, China. E-mail: li6789789@sina.com
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