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| IJCEP Copyright © 2007-All rights reserved. |
| International Journal of Clinical and Experimental Pathology |
Int J Clin Exp Pathol 1(5): 435-439;2008
Original Article
High Endogenous Avidin Binding Activity: An Inexpensive and Readily Available
Marker for the Differential Diagnosis of Kidney Neoplasms
Kazunori Kanehira, Johnny Hu, Thomas Pier, Linda Sebree and Wei Huang
Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, WI 53792, USA; Department of Pathology and
Laboratory Medicine, University of Wisconsin Hospital and Clinics, Madison WI 53792, USA
Received 21 Oct 2007; accepted with revision 5 Nov 2007; available online 1 January 2008
Abstract: It has been documented that some tissues, such as salivary gland, liver, cardiac and skeletal muscles and kidney, have high
level endogenous biotin or endogenous avidin binding activity (EABA). Limited data is available on EABA in renal cell neoplasms. A tissue
microarray (TMA) was constructed that included oncocytoma (n=30), chromophobe renal cell carcinoma (RCC) (n=18), clear cell RCC
(n=45), clear cell RCC with granular/eosinophilic (G/E) features (n=19), papillary RCC (n=21), papillary RCC with G/E features (n=29) and
benign renal tissues (n=31). The TMA slides were stained with or without biotin blocker and analyzed using the automated cellular
imaging system (ACIS®). Without biotin blocker, a high positive rate of EABA was found in oncocytoma (56/60, 93%) and normal renal
tubules (46/60, 77%). A moderate positive rate of EABA was found in clear cell and papillary RCCs with G/E features (13/39, 33% and
19/55, 35%, respectively). Chromophobe RCC and RCC without G/E features had essentially no EABA. With biotin blocker, benign renal
tissue and clear cell RCC were negative for EABA; but a significant number of renal oncocytoma (29/60, 48%) and a few papillary RCC
with G/E features (5/52, 10%) remained positive for EABA. In conclusion, high EABA may be used to differentiate oncocytoma from
chromophobe RCC, and the staining results must be interpreted with caution when avidin-biotin detection system is used in diagnosing
renal neoplasms. (IJCEP710005).
Key Words: Oncocytoma, endogenous avidin binding activity (EABA), chromophobe renal cell carcinoma
Full Text PDF
Address all correspondences to: Wei Huang, MD, Department of Pathology and Laboratory Medicine, University of Wisconsin, 2500
Overlook Terrace, Madison WI 53792. Tel: (608) 262-5787; Email: whuang23@wisc.edu
Original Article
High Endogenous Avidin Binding Activity: An Inexpensive and Readily Available
Marker for the Differential Diagnosis of Kidney Neoplasms
Kazunori Kanehira, Johnny Hu, Thomas Pier, Linda Sebree and Wei Huang
Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, WI 53792, USA; Department of Pathology and
Laboratory Medicine, University of Wisconsin Hospital and Clinics, Madison WI 53792, USA
Received 21 Oct 2007; accepted with revision 5 Nov 2007; available online 1 January 2008
Abstract: It has been documented that some tissues, such as salivary gland, liver, cardiac and skeletal muscles and kidney, have high
level endogenous biotin or endogenous avidin binding activity (EABA). Limited data is available on EABA in renal cell neoplasms. A tissue
microarray (TMA) was constructed that included oncocytoma (n=30), chromophobe renal cell carcinoma (RCC) (n=18), clear cell RCC
(n=45), clear cell RCC with granular/eosinophilic (G/E) features (n=19), papillary RCC (n=21), papillary RCC with G/E features (n=29) and
benign renal tissues (n=31). The TMA slides were stained with or without biotin blocker and analyzed using the automated cellular
imaging system (ACIS®). Without biotin blocker, a high positive rate of EABA was found in oncocytoma (56/60, 93%) and normal renal
tubules (46/60, 77%). A moderate positive rate of EABA was found in clear cell and papillary RCCs with G/E features (13/39, 33% and
19/55, 35%, respectively). Chromophobe RCC and RCC without G/E features had essentially no EABA. With biotin blocker, benign renal
tissue and clear cell RCC were negative for EABA; but a significant number of renal oncocytoma (29/60, 48%) and a few papillary RCC
with G/E features (5/52, 10%) remained positive for EABA. In conclusion, high EABA may be used to differentiate oncocytoma from
chromophobe RCC, and the staining results must be interpreted with caution when avidin-biotin detection system is used in diagnosing
renal neoplasms. (IJCEP710005).
Key Words: Oncocytoma, endogenous avidin binding activity (EABA), chromophobe renal cell carcinoma
Full Text PDF
Address all correspondences to: Wei Huang, MD, Department of Pathology and Laboratory Medicine, University of Wisconsin, 2500
Overlook Terrace, Madison WI 53792. Tel: (608) 262-5787; Email: whuang23@wisc.edu