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| IJCEP Copyright © 2007-All rights reserved. |
| International Journal of Clinical and Experimental Pathology |
Int J Clin Exp Pathol 1(6), 475-488; 2008
Review Article
Laser Capture Microdissection in the Genomic and Proteomic Era: Targeting the
Genetic Basis of Cancer
Barbara Domaze, Gregory T. MacLennan, Antonio Lopez-Beltran, Rodolfo Montironi, and Liang Cheng
Departments of Pathology and Laboratory Medicine and Urology, Indiana University School of Medicine, Indianapolis, IN, USA;
Department of Pathology, Case Western Reserve University, Cleveland, OH, USA; Department of Pathology, Cordoba University, Cordoba,
Spain and Institute of Pathological Anatomy and Histopathology, School of Medicine, Polytechnic University of the Marche Region
(Ancona), United Hospitals, Ancona, Italy
Received 25 Feb 2008; accepted and available online 15 March 2008
Abstract: The advent of new technologies has enabled deeper insight into processes at subcellular levels, which will ultimately improve
diagnostic procedures and patient outcome. Thanks to cell enrichment methods, it is now possible to study cells in their native
environment. This has greatly contributed to a rapid growth in several areas, such as gene expression analysis, proteomics, and
metabolonomics. Laser capture microdissection (LCM) as a method of procuring subpopulations of cells under direct visual inspection is
playing an important role in these areas. This review provides an overview of existing LCM technology and its downstream applications in
genomics, proteomics, diagnostics and therapy. (IJCEP802002).
Key Words: Laser capture microdissection, genomics, proteomics, carcinogenesis, clonality, biomarker discovery, prognosis
Full Text PDF
Address all correspondences to: Liang Cheng, MD, Department of Pathology and Laboratory Medicine, Indiana University School of
Medicine, 350 West 11th Street, Clarian Pathology Laboratory Room 4010, Indianapolis, IN 46202, USA. Telephone: 317-491-6442; Fax:
317-491-6419; Email: liang_cheng@yahoo.com
Review Article
Laser Capture Microdissection in the Genomic and Proteomic Era: Targeting the
Genetic Basis of Cancer
Barbara Domaze, Gregory T. MacLennan, Antonio Lopez-Beltran, Rodolfo Montironi, and Liang Cheng
Departments of Pathology and Laboratory Medicine and Urology, Indiana University School of Medicine, Indianapolis, IN, USA;
Department of Pathology, Case Western Reserve University, Cleveland, OH, USA; Department of Pathology, Cordoba University, Cordoba,
Spain and Institute of Pathological Anatomy and Histopathology, School of Medicine, Polytechnic University of the Marche Region
(Ancona), United Hospitals, Ancona, Italy
Received 25 Feb 2008; accepted and available online 15 March 2008
Abstract: The advent of new technologies has enabled deeper insight into processes at subcellular levels, which will ultimately improve
diagnostic procedures and patient outcome. Thanks to cell enrichment methods, it is now possible to study cells in their native
environment. This has greatly contributed to a rapid growth in several areas, such as gene expression analysis, proteomics, and
metabolonomics. Laser capture microdissection (LCM) as a method of procuring subpopulations of cells under direct visual inspection is
playing an important role in these areas. This review provides an overview of existing LCM technology and its downstream applications in
genomics, proteomics, diagnostics and therapy. (IJCEP802002).
Key Words: Laser capture microdissection, genomics, proteomics, carcinogenesis, clonality, biomarker discovery, prognosis
Full Text PDF
Address all correspondences to: Liang Cheng, MD, Department of Pathology and Laboratory Medicine, Indiana University School of
Medicine, 350 West 11th Street, Clarian Pathology Laboratory Room 4010, Indianapolis, IN 46202, USA. Telephone: 317-491-6442; Fax:
317-491-6419; Email: liang_cheng@yahoo.com