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Int J Clin Exp Pathol 2(5),519-527;2009
Original Article
MicroRNA Expression Profiling Outperforms mRNA Expression Profiling in
Formalin-fixed Paraffin-embedded Tissues
Aihua Liu, Michael T. Tetzlaff, Pat VanBelle, David Elder, Michael Feldman, John W. Tobias, Antonio Sepulveda and Xiaowei Xu
Department of Pathology and Laboratory Medicine, 6 Founders Pavilion, The Hospital for the University of Pennsylvania, 3400 Spruce Street,
Philadelphia, PA 19040
Received 03 March 2009; Accepted and Available online 20 March 2009
Abstract: microRNAs (miRNAs) are ~22nt RNAs that regulate target gene expression. Altered expression of miRNAs has been demonstrated
in many different human cancers. Many studies using microarray technologies to characterize miRNA expression profiles have relied on fresh
tissue to determine the miRNA signatures. In this study, we prepared total RNA from paired samples of formalin-fixed paraffin-embedded
(FFPE) and fresh frozen malignant melanoma, and used that in microarray experiments to compare miRNA expression profiles between FFPE
and fresh tissue with corresponding mRNA expression profiles from the same tissue sources. We demonstrate that miRNA expression profile
from FFPE tissues closely resembles that from fresh tissues, and the correlation is significantly better than that for mRNA profiles from FFPE
and fresh tissues. These results underscore the suitability of FFPE tissues as appropriate resources for molecular expression analyses and
support the notion that miRNAs are more vigorous analytes for this purpose than mRNAs. (IJCEP903002).
Key Words: miRNA, melanoma, formalin-fixed paraffin-embedded (FFPE), microarray
Full text PDF
Address all correspondence to: Xiaowei Xu, MD, PhD, Department of Pathology and Laboratory Medicine, University of Pennsylvania School of
Medicine, 3400 Spruce Street. Philadelphia, PA 19104. Tel: 215-662-6503, Fax: 215-649-5910; Email: xug@mail.med.upenn.edu
Original Article
MicroRNA Expression Profiling Outperforms mRNA Expression Profiling in
Formalin-fixed Paraffin-embedded Tissues
Aihua Liu, Michael T. Tetzlaff, Pat VanBelle, David Elder, Michael Feldman, John W. Tobias, Antonio Sepulveda and Xiaowei Xu
Department of Pathology and Laboratory Medicine, 6 Founders Pavilion, The Hospital for the University of Pennsylvania, 3400 Spruce Street,
Philadelphia, PA 19040
Received 03 March 2009; Accepted and Available online 20 March 2009
Abstract: microRNAs (miRNAs) are ~22nt RNAs that regulate target gene expression. Altered expression of miRNAs has been demonstrated
in many different human cancers. Many studies using microarray technologies to characterize miRNA expression profiles have relied on fresh
tissue to determine the miRNA signatures. In this study, we prepared total RNA from paired samples of formalin-fixed paraffin-embedded
(FFPE) and fresh frozen malignant melanoma, and used that in microarray experiments to compare miRNA expression profiles between FFPE
and fresh tissue with corresponding mRNA expression profiles from the same tissue sources. We demonstrate that miRNA expression profile
from FFPE tissues closely resembles that from fresh tissues, and the correlation is significantly better than that for mRNA profiles from FFPE
and fresh tissues. These results underscore the suitability of FFPE tissues as appropriate resources for molecular expression analyses and
support the notion that miRNAs are more vigorous analytes for this purpose than mRNAs. (IJCEP903002).
Key Words: miRNA, melanoma, formalin-fixed paraffin-embedded (FFPE), microarray
Full text PDF
Address all correspondence to: Xiaowei Xu, MD, PhD, Department of Pathology and Laboratory Medicine, University of Pennsylvania School of
Medicine, 3400 Spruce Street. Philadelphia, PA 19104. Tel: 215-662-6503, Fax: 215-649-5910; Email: xug@mail.med.upenn.edu
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