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Int J Clin Exp Pathol 2010;3(1):87-97
Original Article
Columnar Cell lesions and Pseudoangiomatous hyperplasia like stroma: Is there an
epithelial-stromal interaction?
Rosemary A. Recavarren, Mamatha Chivukula, Gloria Carter, David J. Dabbs
Department of Pathology at Magee Women’s Hospital of University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA.
Received March 12, 2009; Accepted August 18, 2008; Available online August 30, 2009
Abstract: The significance of association between cancer and its microenvironment has been increasingly recognized. It has been shown in
animal models that interaction between neoplastic epithelial cells and adjacent stroma can modulate tumor behavior. Carcinoma associated
stromal cells can transform normal epithelial cells into neoplastic cells. In breast, columnar cell lesions are non-obligate precursors of low
grade ductal carcinoma in situ. Columnar cell lesions can be seen intimately associated with PASH-like-stroma, a lesion we termed as
CCPLS. Our aim is to investigate epithelial-stromal interactions in CCPLS and compare them to PASH without columnar cell lesions in breast
core needle biopsies. Normal terminal duct lobular unit (TDLU) epithelium was seen in association with columnar cell lesions as well as
PASH. Eight (8) cases of each category were examined by a panel of immunostains: CD117 (C-kit), CD34, CD105, bFGF, AR, ER-beta, MIB-1.
We observed a markedly decreased expression of c-kit in columnar cell lesions compared to TDLU-epithelium. CD105 showed a quantitative
increase in activated vessels in CCPLS compared to PASH. A subset of CCPLS and PASH were androgen receptor positive. A strong nuclear
positivity for ER-beta is observed in the epithelium and stroma of all CCPLS cases. We conclude that (1) activated blood vessels predominate
in CCPLS; (2) A molecular alteration is signified by c-kit loss in columnar cell lesions; (3) ER-beta and androgen receptor positivity indicate
CCPLS are hormonally responsive lesions. Our study suggests an intimate vascular and hormone dependent epithelial-stromal interaction
exists in CCPLS lesions. (IJCEP903005).
Key Words: Columnar cell, Pseudoangiomatous stromal hyperplasia, c-kit, AR, ER-beta
Full text PDF
Address all correspondence to:
Mamatha Chivukula, MD
Department of Pathology at Magee Women’s Hospital
University of Pittsburgh Medical Center
Pittsburgh, Pennsylvania
USA.
E-mail: mchivukula@mail.magee.edu
Original Article
Columnar Cell lesions and Pseudoangiomatous hyperplasia like stroma: Is there an
epithelial-stromal interaction?
Rosemary A. Recavarren, Mamatha Chivukula, Gloria Carter, David J. Dabbs
Department of Pathology at Magee Women’s Hospital of University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA.
Received March 12, 2009; Accepted August 18, 2008; Available online August 30, 2009
Abstract: The significance of association between cancer and its microenvironment has been increasingly recognized. It has been shown in
animal models that interaction between neoplastic epithelial cells and adjacent stroma can modulate tumor behavior. Carcinoma associated
stromal cells can transform normal epithelial cells into neoplastic cells. In breast, columnar cell lesions are non-obligate precursors of low
grade ductal carcinoma in situ. Columnar cell lesions can be seen intimately associated with PASH-like-stroma, a lesion we termed as
CCPLS. Our aim is to investigate epithelial-stromal interactions in CCPLS and compare them to PASH without columnar cell lesions in breast
core needle biopsies. Normal terminal duct lobular unit (TDLU) epithelium was seen in association with columnar cell lesions as well as
PASH. Eight (8) cases of each category were examined by a panel of immunostains: CD117 (C-kit), CD34, CD105, bFGF, AR, ER-beta, MIB-1.
We observed a markedly decreased expression of c-kit in columnar cell lesions compared to TDLU-epithelium. CD105 showed a quantitative
increase in activated vessels in CCPLS compared to PASH. A subset of CCPLS and PASH were androgen receptor positive. A strong nuclear
positivity for ER-beta is observed in the epithelium and stroma of all CCPLS cases. We conclude that (1) activated blood vessels predominate
in CCPLS; (2) A molecular alteration is signified by c-kit loss in columnar cell lesions; (3) ER-beta and androgen receptor positivity indicate
CCPLS are hormonally responsive lesions. Our study suggests an intimate vascular and hormone dependent epithelial-stromal interaction
exists in CCPLS lesions. (IJCEP903005).
Key Words: Columnar cell, Pseudoangiomatous stromal hyperplasia, c-kit, AR, ER-beta
Full text PDF
Address all correspondence to:
Mamatha Chivukula, MD
Department of Pathology at Magee Women’s Hospital
University of Pittsburgh Medical Center
Pittsburgh, Pennsylvania
USA.
E-mail: mchivukula@mail.magee.edu
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