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Int J Clin Exp Pathol 2010;3(1):98-105
Original Article
Utility of desmin and a Masson’s trichrome method to detect early acute myocardial
infarction in autopsy tissues
Jie Ouyang, Miguel Guzman, Fidelina Desoto-Lapaix, Matthew R.Pincus, Rosemary Wieczorek
Department of Pathology, State University of New York Downstate Medical Center, and New York harbor Healthcare System, Brooklyn, NY
Received May 27, 2009; accepted July 21, 2009; Available online September 20, 2009
Abstract: Detection of early acute myocardial ischemia/infarction prior to neutrophilic infiltration in autopsy myocardium poses a diagnostic
dilemma to the surgical pathologist. Morphological changes can be subtle or not identified at all on the hematoxylin and eosin stain.To
evaluate the Masson’s trichrome stain and immunohistochemical stains, desmin and myoglobin, in detecting acute myocardial
ischemia/infarction in autopsy myocardium.We reviewed the autopsy files of the New York Harbor Healthcare System and retrieved 25 cases of
early acute myocardial infarction. Three autopsy hearts of non-cardiac related deaths were used as controls. Sections from grossly suspected
early acute myocardial infaction areas were stained by a Masson’s trichrome stain technique and with desmin by a standard
immnunohistochemical method.The ischemic zone surrounding myocardial infarction and the acute infarct itself in 23/25(92%) were detected
by desmin depletion, and in all cases with Masson’s trichrome color changes. No change in staining for desmin or Masson’s trichrome were
seen in the three controls. Desmin and Masson’s trichrome together are valuable tools when faced with the problem of postmortem detection
of early myocardial infarction/ischemia. (IJCEP905004).
Key words: Desmin, Masson’s trichrome stain, myocardial infarction, autopsy
Full text PDF
Address all correspondence to:
Rosemary Wieczorek, MD
New York harbor Healthcare System
100 Poly Place, Room3-305
Brooklyn, NY 11209
Email: Rosemary.Wieczorek@va.gov
Original Article
Utility of desmin and a Masson’s trichrome method to detect early acute myocardial
infarction in autopsy tissues
Jie Ouyang, Miguel Guzman, Fidelina Desoto-Lapaix, Matthew R.Pincus, Rosemary Wieczorek
Department of Pathology, State University of New York Downstate Medical Center, and New York harbor Healthcare System, Brooklyn, NY
Received May 27, 2009; accepted July 21, 2009; Available online September 20, 2009
Abstract: Detection of early acute myocardial ischemia/infarction prior to neutrophilic infiltration in autopsy myocardium poses a diagnostic
dilemma to the surgical pathologist. Morphological changes can be subtle or not identified at all on the hematoxylin and eosin stain.To
evaluate the Masson’s trichrome stain and immunohistochemical stains, desmin and myoglobin, in detecting acute myocardial
ischemia/infarction in autopsy myocardium.We reviewed the autopsy files of the New York Harbor Healthcare System and retrieved 25 cases of
early acute myocardial infarction. Three autopsy hearts of non-cardiac related deaths were used as controls. Sections from grossly suspected
early acute myocardial infaction areas were stained by a Masson’s trichrome stain technique and with desmin by a standard
immnunohistochemical method.The ischemic zone surrounding myocardial infarction and the acute infarct itself in 23/25(92%) were detected
by desmin depletion, and in all cases with Masson’s trichrome color changes. No change in staining for desmin or Masson’s trichrome were
seen in the three controls. Desmin and Masson’s trichrome together are valuable tools when faced with the problem of postmortem detection
of early myocardial infarction/ischemia. (IJCEP905004).
Key words: Desmin, Masson’s trichrome stain, myocardial infarction, autopsy
Full text PDF
Address all correspondence to:
Rosemary Wieczorek, MD
New York harbor Healthcare System
100 Poly Place, Room3-305
Brooklyn, NY 11209
Email: Rosemary.Wieczorek@va.gov
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